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. 2018 Mar 27;46(8):4087–4098. doi: 10.1093/nar/gky219

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — A model of conformation states of Cascade at different target variants. The Cse1 subunit is predominantly in the open state for the –1 and +1 substitutions resulting in a weak (semi-locked) R-loop that is not able to support efficient Cas3 recruitment. For other seed substitutions the locking strength and the ability to recruit Cas3 increase with increasing distance from the PAM, reaching the WT level for +4 substitution (suggesting a closed WT-like Cse1 conformation that readily recruits Cas3).