Table 2. Photophysical properties of the pA nucleoside monomer (1) in different solvents.
Solvent | λ Abs,1 (nm) | ε 1 (M–1 cm–1) | λ Abs,2 (nm) | ε 2 (M–1 cm–1) | λ Em (nm) | Φ F (%) | ε 1 Φ F a (M–1 cm–1) |
Water b | 387 | 15 200 | 368 | 11 400 | 420 | 66 | 10 100 |
DMSO | 396 | 17 100 | 378 | 12 500 | 421 | 84 | 14 400 |
EtOH | 390 | 17 500 | 370 | 12 600 | 405 | 66 | 11 600 |
DCM | 392 | 16 100 | 373 | 11 700 | 410 | 74 | 11 900 |
Toluene | 397 | 18 700 | 377 | 13 200 | 409 | 72 | 13 400 |
MeCN | 390 | 16 500 | 372 | 11 900 | 414 | 64 | 10 500 |
aBrightness is calculated as the product of the fluorescence quantum yield (ΦF) and the molar absorptivity at the long-wavelength maximum (ε1).
bWith 2% DMSO.