Table 4. Photophysical properties of single- and double-stranded DNA sequences containing pA.
NN a | Single strands |
Double strands |
||||||
ε Abs b (M–1 cm–1) | λ Em (nm) | Φ F c (%) | εΦ F (M–1 cm–1) | ε Abs b (M–1 cm–1) | λ Em (nm) | Φ F c (%) | εΦ F (M–1 cm–1) | |
AA | 14 500 | 407 | 58 | 8400 | 13 800 | 403 | 22 | 3000 |
AC | 15 000 | 406 | 11 | 1700 | 14 300 | 402 | 6.2 | 890 |
AG | 15 200 | 405 | 24 | 3600 | 13 200 | 403 | 16 | 2100 |
AT | 15 000 | 407 | 15 | 2300 | 14 500 | 400 | 17 | 2500 |
CA | 14 200 | 408 | 14 | 2000 | 12 600 | 405 | 9.9 | 1200 |
CC | 14 500 | 407 | 3.6 | 520 | 12 900 | 406 | 4.3 | 560 |
CG | 14 700 | 405 | 6.1 | 900 | 12 400 | 406 | 8.2 | 1000 |
CT | 14 000 | 409 | 4.1 | 570 | 13 500 | 403 | 10 | 1400 |
GA | 15 000 | 407 | 42 | 6300 | 14 000 | 404 | 14 | 2000 |
GC | 16 000 | 404 | 4.4 | 700 | 14 700 | 405 | 3.4 | 500 |
GG | 14 300 | 406 | 28 | 4000 | 13 200 | 405 | 13 | 1700 |
GT | 15 200 | 407 | 8.3 | 1300 | 14 400 | 401 | 7.7 | 1100 |
TA | 14 100 | 408 | 6.2 | 870 | 13 300 | 402 | 8.0 | 1100 |
TC | 14 400 | 406 | 2.4 | 350 | 13 000 | 403 | 4.4 | 570 |
TG | 14 500 | 408 | 3.3 | 480 | 12 600 | 404 | 6.7 | 840 |
TT | 14 100 | 409 | 2.6 | 370 | 13 600 | 400 | 11 | 1500 |
aSequences are named according to nucleotides surrounding pA; full sequences can be found in Table 3.
bMolar absorptivity values are reported as an average of two or more experiments with a standard error of ≤200.
cQuantum yields were determined for the emission profiles shown in Fig. S3, with quinine sulfate as reference (ΦF = 54.6% in 0.5 M H2SO4), using an excitation wavelength of 353 nm, and are reported as an average of two or more experiments with a standard error of ≤0.8%.