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. 2018 Mar 1;9(14):3494–3502. doi: 10.1039/c7sc05448c

Table 4. Photophysical properties of single- and double-stranded DNA sequences containing pA.

NN a Single strands
Double strands
ε Abs b (M–1 cm–1) λ Em (nm) Φ F c (%) εΦ F (M–1 cm–1) ε Abs b (M–1 cm–1) λ Em (nm) Φ F c (%) εΦ F (M–1 cm–1)
AA 14 500 407 58 8400 13 800 403 22 3000
AC 15 000 406 11 1700 14 300 402 6.2 890
AG 15 200 405 24 3600 13 200 403 16 2100
AT 15 000 407 15 2300 14 500 400 17 2500
CA 14 200 408 14 2000 12 600 405 9.9 1200
CC 14 500 407 3.6 520 12 900 406 4.3 560
CG 14 700 405 6.1 900 12 400 406 8.2 1000
CT 14 000 409 4.1 570 13 500 403 10 1400
GA 15 000 407 42 6300 14 000 404 14 2000
GC 16 000 404 4.4 700 14 700 405 3.4 500
GG 14 300 406 28 4000 13 200 405 13 1700
GT 15 200 407 8.3 1300 14 400 401 7.7 1100
TA 14 100 408 6.2 870 13 300 402 8.0 1100
TC 14 400 406 2.4 350 13 000 403 4.4 570
TG 14 500 408 3.3 480 12 600 404 6.7 840
TT 14 100 409 2.6 370 13 600 400 11 1500

aSequences are named according to nucleotides surrounding pA; full sequences can be found in Table 3.

bMolar absorptivity values are reported as an average of two or more experiments with a standard error of ≤200.

cQuantum yields were determined for the emission profiles shown in Fig. S3, with quinine sulfate as reference (ΦF = 54.6% in 0.5 M H2SO4), using an excitation wavelength of 353 nm, and are reported as an average of two or more experiments with a standard error of ≤0.8%.