Table 5. Synthesized 33-mer sequences used for interbase FRET measurements.
Sequence name a | DNA sequence b | Φ F c (%) |
D7 | 5′-d(CGA TCA (pA)AA AAA ATT W)-3′ | 20 |
D9 | 5′-d(CGA TCA AA(pA) AAA ATT W)-3′ | 24 |
D11 | 5′-d(CGA TCA AAA A(pA)A ATT W)-3′ | 28 |
A0 | 5′-d(X TAT AAT CGT AAT TTT Z)-3′ | |
A13 | 5′-d(X TAT qAnitro AT CGT AAT TTT Z)-3′ | |
A14 | 5′-d(X TAT A qAnitro T CGT AAT TTT Z)-3′ | |
A19 | 5′-d(X TAT AAT CGT qAnitro AT TTT Z)-3′ | |
A20 | 5′-d(X TAT AAT CGT A qAnitro T TTT Z)-3′ |
aSequence and sample preparation can be found in the ESI. Samples are named by the donor (D) or acceptor (A) position from the 5′ end.
b W = ACG ATT ATA AGG AGG AGG. X = CCT CCT CCT; Z = TTT TGA TCG.
cQuantum yields were determined with quinine sulfate as reference (ΦF = 54.6% in 0.5 M H2SO4) using an excitation wavelength of 353 nm.