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. 2018 Mar 1;9(14):3494–3502. doi: 10.1039/c7sc05448c

Table 5. Synthesized 33-mer sequences used for interbase FRET measurements.

Sequence name a DNA sequence b Φ F c (%)
D7 5′-d(CGA TCA (pA)AA AAA ATT W)-3′ 20
D9 5′-d(CGA TCA AA(pA) AAA ATT W)-3′ 24
D11 5′-d(CGA TCA AAA A(pA)A ATT W)-3′ 28
A0 5′-d(X TAT AAT CGT AAT TTT Z)-3′
A13 5′-d(X TAT qAnitro AT CGT AAT TTT Z)-3′
A14 5′-d(X TAT A qAnitro T CGT AAT TTT Z)-3′
A19 5′-d(X TAT AAT CGT qAnitro AT TTT Z)-3′
A20 5′-d(X TAT AAT CGT A qAnitro T TTT Z)-3′

aSequence and sample preparation can be found in the ESI. Samples are named by the donor (D) or acceptor (A) position from the 5′ end.

b W = ACG ATT ATA AGG AGG AGG. X = CCT CCT CCT; Z = TTT TGA TCG.

cQuantum yields were determined with quinine sulfate as reference (ΦF = 54.6% in 0.5 M H2SO4) using an excitation wavelength of 353 nm.