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. 2018 Mar 15;15(5):7523–7530. doi: 10.3892/ol.2018.8266

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Copyright: © Ye et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Downregulation of AFAP1-AS1 inhibits GC cell proliferation ability. (A) RT-qPCR was performed to analyze the expression level of AFAP1-AS1 in SGC-7901, BGC-823 and MGC-803 GC cell lines and GES-1 human gastric epithelial cell line. (B) sh1AFAP1-AS1 and sh2AFAP1-AS1, shRNA expression vectors targeting AFAP1-AS1, and a negative control shRNA vector were transfected into SGC-7901 and BGC-823 cells, respectively. RT-qPCR results revealed that the sh1AFAP1-AS1 sequence had the greatest inhibitory effect. (C) Cell Counting Kit-8 assays were performed to examine SGC-7901 and BGC-823 cell proliferation rates at the indicated time points. The results were analyzed using one-way analysis of variance and Student's t-test, *P<0.05. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; AFAP1-AS1, AFAP1-antisense RNA 1; GC, gastric cancer; sh, short hairpin; shNC, scrambled control shRNA.