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. 2018 Mar 15;15(5):7523–7530. doi: 10.3892/ol.2018.8266

Table I.

Sequences of the insertions in vectors and primers.

Name Sequence
Insertion vector
  sh1AFAP1-AS1 F: 5′-CCGGAACACCAATCCCAAGAGGTGACTCGAGTCACCTCTTGGGATTGGTGTTTTTTTG-3′
R: 5′-AATTCAAAAAAACACCAATCCCAAGAGGTGACTCGAGTCACCTCTTGGGATTGGTGTT-3′
  sh2AFAP1-AS1 F: 5′-CCGGAGCGGTCTCAGCCGAATGACTCTCGAGAGTCATTCGGCTGAGACCGCTTTTTTG-3′
R: 5′-AATTCAAAAAAGCGGTCTCAGCCGAATGACTCTCGAGAGTCATTCGGCTGAGACCGCT-3′
  shNC F: 5′-CCGGTTTCTCCGAACGTGTCACGTCTCGAGACGTGACACGTTCGGAGAATTTTTG-3′
R: 5′-AATTCAAAAAGTTCTCCGAACGTGTCACGTCTCGAGACGTGACACGTTCGGAGAA-3′
Primer
  AFAP1-AS1 F: 5′-TCGCTCAATGGAGTGACGGCA-3′
R: 5′-CGGCTGAGACCGCTGAGAACTT-3′
  GAPDH F: 5′-TTAGTCGTCAATGGCAACAAG-3′
R: 5′-GACACCGACGACGAACATAG-3′

sh, short hairpin; AFAP1-AS1, AFAP1-antisense RNA 1; shNC, scrambled control shRNA; F, forward; R, reverse.