Table I.
Name | Sequence |
---|---|
Insertion vector | |
sh1AFAP1-AS1 | F: 5′-CCGGAACACCAATCCCAAGAGGTGACTCGAGTCACCTCTTGGGATTGGTGTTTTTTTG-3′ |
R: 5′-AATTCAAAAAAACACCAATCCCAAGAGGTGACTCGAGTCACCTCTTGGGATTGGTGTT-3′ | |
sh2AFAP1-AS1 | F: 5′-CCGGAGCGGTCTCAGCCGAATGACTCTCGAGAGTCATTCGGCTGAGACCGCTTTTTTG-3′ |
R: 5′-AATTCAAAAAAGCGGTCTCAGCCGAATGACTCTCGAGAGTCATTCGGCTGAGACCGCT-3′ | |
shNC | F: 5′-CCGGTTTCTCCGAACGTGTCACGTCTCGAGACGTGACACGTTCGGAGAATTTTTG-3′ |
R: 5′-AATTCAAAAAGTTCTCCGAACGTGTCACGTCTCGAGACGTGACACGTTCGGAGAA-3′ | |
Primer | |
AFAP1-AS1 | F: 5′-TCGCTCAATGGAGTGACGGCA-3′ |
R: 5′-CGGCTGAGACCGCTGAGAACTT-3′ | |
GAPDH | F: 5′-TTAGTCGTCAATGGCAACAAG-3′ |
R: 5′-GACACCGACGACGAACATAG-3′ |
sh, short hairpin; AFAP1-AS1, AFAP1-antisense RNA 1; shNC, scrambled control shRNA; F, forward; R, reverse.