Figure 3.

Effects of miR-433 expression on the migration and invasion of colorectal cancer cells. (A) Morphology of tumor cells. Colorectal cancer LoVo cells were grown and transiently transfected with miR-433 mimics or negative control. The cells were subsequently viewed under a bright-phase microscope. The data indicated following transfection of miR-433 mimic, LoVo cells exhibited a rounded/cobble-stone morphology. By contrast, LoVo cells exhibited a more elongated morphology. Scale bar, 50 µm. (B) Transwell migration assay. Colorectal cancer LoVo cells were grown and transiently transfected with miR-433 mimics or negative control. The cells were subjected to Transwell assay. Scale bar, 50 µm. (C) Transwell invasion assay. Colorectal cancer LoVo cells were grown and transiently transfected with miR-433 mimics or negative control and then subjected to the Transwell invasion assay. Scale bar, 50 µm. (D) Wound-healing assay. Colorectal cancer LoVo cells were grown and transiently transfected with miR-433 mimics or negative control and then subjected to a wound-healing assay. Scale bar, 200 µm. (E) Western blot results. Colorectal cancer LoVo cells were grown and transiently transfected with miR-433 mimics or negative control and then subjected to western blot analysis for E-cadherin, N-cadherin, vimentin, and fibronectin. E-ca, E-cadherin; FN1, fibronectin; N-ca, N-cadherin; VIM, vimentin; miR, microRNA; NC, negative control. *P<0.05 with comparisons shown by lines.