Figure 3.

DpC induces nuclear damage of Cal-27. The change in nuclear morphology by DpC on the Cal-27 cell line was detected by Hoechst staining. The experimental results showed that upon treatment with a range of concentrations of DpC (0, 2.5, 5 and 7.5 µM). (A) The nuclear staining of the Cal-27 cells when treated with 0 µM DpC. (B) The nuclear staining of the Cal-27 cells when treated with 2.5 µM DpC. (C) The nuclear staining of the Cal-27 cells when treated with 5 µM DpC. (D) The nuclear staining of the Cal-27 cells when treated with 7.5 µM DpC; the proportion of nuclei with bright blue fluorescence decreased significantly. DpC, di-2-pyridylketone-4-cyclohexyl-4-methyl-3-thiosemicarbazone (magnification, ×40).