Table 2.

K_D values for aptamers LDHp 1, LDHp 11, rLDH 4, rLDH 7, rLDH 15, C7 and pL1 binding to immobilized proteins, rPfLDH and rPvLDH determined using ELONA

Aptamer	Apparent affinity constant, KD (nM) ± SE				References
	rPfLDH		rPvLDH
	KD	Γmax	KD	Γmax
C7	NR	NR	NR	NR	This work
LDHp 1	927.3 ± 915.0	0.068 ± 0.039	CNM > 1000	0.230 ± 0.106	This work
LDHp 11	321.2 ± 82.5*	0.135 ± 0.014*	37.0 ± 41.4 CNM	0.097 ± 0.020*	This work
rLDH 4	691.6 ± 393.6	0.191 ± 0.057*	444. 9 ± 144.3*	0.364 ± 0.049*	This work
rLDH 7	39.9 ± 15.7*	0.180 ± 0.014*	26.3 ± 3.2*	0.283 ± 0.050*	This work
rLDH 15	80.7 ± 17.1*	0.129 ± 0.007*	268.7 ± 67.2*	0.501 ± 0.429*	This work
pL1	159.5 ± 167.8 CNM	0.022 ± 0.005*	79.2 ± 12.7*	0.209 ± 0.010*	This work
	38.7 ± 1.3	–	16.8 ± 0.6	–	[20]
	6.2	–	2.9	–	[24]
2008s	42.0–59.0	–	–	–	[23]
	43.0	–	NR	–	[24]

K_D estimated apparent dissociation constant (M) of the aptamer-target complex, Γ_max estimated maximal assay response for the aptamer-target complex, CNM could not model—positive binding occurred, but no valid modelled K_D was obtained, CNM > 1000 could not model—linear dependence indicates that apparent K_D of a target did not fall within the tested concentration range i.e. K_D > 1000 nM, NR no response—no evidence of binding, relative to the baseline assay response

* Wald test produced a probability, p of < 0.05 for this parameter