Table 1.
Strains and plasmids used in this study
| Strain/plasmid | Description | References |
|---|---|---|
| Strains | ||
| E. coli TOP10 | F–mcrA Δ (mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ (ara leu) 7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
| E. coli S17-1 λpir | Donor strain. TpR SmR recA, thi, pro, hsd (R-M+) RP4: 2-Tc:Mu: Km Tn7 λpir | [27] |
| M. buryatense 5GB1 | Moderately haloalkaliphilic methanotroph | [17] |
| M. buryatense 5GB1 ∆glgA1 ∆glgA2 | M. buryatense 5GB1 ∆glgA1 ∆glgA2 | [17] |
| AP18 | M. buryatense 5GB1C [17] ∆glgA1 ∆glgA2 ∆sps | This study |
| Plasmids | ||
| pAWP93 | pCM433kanT containing flanks to knock out repA-repB locus of native plasmid. | [17] |
| pAB2 | Kanamycin-resistant variant of pCM433 containing flanks to knock out sps | This study |