Table 2.

Primers used in this study

Primer name	Sequence (5′–3′)	Description
AP186_pCM433kanT_fwd1	ATGTGCAGGTTGTCGGTGTC	For amplifying the backbone of the KanR version of the sucrose counterselection plasmid pCM433 [17]
AP187_pCM433kanT_rev1	TGGTAACTGTCAGACCAAGTTTACTC
AP110_spsKO_UP_Fwd	ATTGGTACCATGGATGCATATGCTGCAGCTACGCTGCTCTAAATACCTTG	For amplifying flanks to knock out sps (MaGE locus tag MBURv2_130613) using plasmid pAB2
AP111_spsKO_UP_Rev	TCTGCTCTCAGTCACGATCGTACTCACTTCAGCTG
AP112_spsKO_DWN_Fwd	GAAGTGAGTACGATCGTGACTGAGAGCAGAATTATGACTG
AP113_spsKO_DWN_Rev	AGTGAGCTCACCGGTTAACACGCGTACGTACCTTCGGCCAAATACAACGA

Homology regions used for Gibson assembly are underlined