Fig. 2.

IL17A mediates MCP230-exacerbated pulmonary neutrophilic inflammation in asthma. a Schematic representing the protocol followed to induce inflammation in a mouse model of asthma. WT and IL17Ra^−/− mice were sensitized with OVA (Ovalbumin complexed to Imject Alum) on days 0 and 14. Mice were exposed to either vehicle or MCP230 (50 μg) on protocol day 23 and challenged with OVA on days 24, 25, and 26. BALF or lungs were collected on day 28. b Differential cell counts of BALF cells at 5 days post-exposure (i.e. day 28) from mice challenged with OVA and exposed to vehicle or MCP230. Data are represented as percentage of total BALF cells. Data represent mean ± SEM from 4 to 5 mice. ^ap < 0.05, compared to WT OVA group. ^bp < 0.05, compared to WT OVA+MCP230 group, one-way ANOVA with Holm-Sidak’s multiple comparisons test. c Representative photomicrographs of the lungs of WT and IL17Ra^−/− mice challenged with OVA and exposed to vehicle or MCP230. Arrowheads point to areas of peribronchiolar and perivascular inflammation. d Quantification of area of inflammation (μm²) surrounding the airways. Data represent mean ± SEM from 4 to 5 mice. ^ap < 0.05, compared to WT OVA group. ^bp < 0.05, compared to WT OVA+MCP230, one-way ANOVA with Tukey's multiple comparisons test