Fig. 2.

CIN substrates show higher abundance in adherent cell lines cultured in serum-containing medium than in non-adherent lines cultured in serum-free medium. a DESeq2 analysis of non-adherent glioblastoma cells cultured in serum-free medium vs. adherent, serum-cultured cell lines. AOX1, the enzyme that converts pyridoxal (the precursor of active pyridoxal 5′-phosphate) to 4-pyridoxate is strongly downregulated in cells cultured in serum-free medium (DESeq2, adjusted p-value< 0.001). Shown is the log2FC + standard error. b Quantification of active vitamin B6 levels in (n = 5 each) serum-cultured lines and cells cultured in serum-free medium. Active vitamin B6 levels are significantly higher (two-sided Mann-Whitney test, p < 0.01) in serum-cultured cell lines. c PhosTag western blot of glioblastoma cells grown under non-adherent, serum-free conditions and adherent, serum-cultured cell lines probed with α-cofilin antibody. d Quantification of n = 5 serum-cultured cell lines and n = 5 cell lines grown in serum-free medium. There is a significant increase (two-sided t-test, p < 0.01) in the p-cofilin/total cofilin ratio in serum-cultured cell lines