Table 2.
Oligonucleotide primers of melting curve-based multiplex real-time PCR
| Targeta | Nucleotide sequence (5′ to 3′) | Amplicon size (bp) | Reference |
|---|---|---|---|
| cfb b | F: CACACATGCTGTTGGAGTTCAGTTGA | 138 | This study |
| R: ACGAAGTCGACAGCATCACACGAAA | |||
| erm(A)/(TR) | F: CCGGCAAGGAGAAGGTTATAATGA | 190 | Otaguiri et al. [5] |
| R: GCATTCACCCGTTGACTCATTTCC | |||
| erm(B) | F: GCTCTTGCACACTCAAGTCTCGAT | 117 | Otaguiri et al. [5] |
| R: ACATCTGTGGTATGGCGGGTAAGT | |||
| mef(A/E) | F: GCGATGGTCTTGTCTATGGCTTCA | 225 | Otaguiri et al. [5] |
| R: AGCTGTTCCAATGCTACGGAT | |||
| RNaseP | F: AGATTTGGACCTGCGAGCG | 64 | WHO [27] |
| R: GAGCGGCTGTCTCCACAAGT | |||
| IGS1 | F: GTCATTTCAGCTGGCGCCATCGATAC | 260 | Tavares et al. [32] |
| R: TTGCCGCATAACGCATCTTAGCCA |
acfb gene encodes the CAMP factor; erm genes encode 23S rRNA methylases; mef gene encodes efflux pumps; RNaseP gene encodes human ribonuclease P; IGS1, intergenic spacer 1 of ribosomal RNA gene cluster of Cryptococcus gattii. bThe nucleotide sequences of Streptococcus agalactiae genes deposited in the GenBank/EMBL databases were used for specific primer design