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. 2018 May 3;18:519. doi: 10.1186/s12885-018-4454-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Overexpression of GFP-tagged Nup88 in HeLa cells. Stable transfectants harboring genes encoding GFP or GFP-fused NUP88 under the control of a tetracycline-inducible system were incubated in medium without (−) or with (+) DOX for 2 days to stimulate protein overexpression. Overexpression and distribution pattern of the proteins in cells were assessed by immunoblotting (a) and by indirect immunofluorescence analysis (b), respectively