Fig. 6.

Overexpression of Nup88 inhibits vimentin dephosphorylation. Schematic of cell lysates preparation from cells arrested in mitosis (a). Mitotic cell lysates containing GFP or Nup88-GFP were incubated in the absence and presence of λPPase for 10 min, followed by detecting phospho-vimentin (pVimentin) by immunoblotting assay (b). Decrease of pVimentin in the absence of λPPase corresponding to Fig. 6b lanes 1-4 (c). A decrease of pVimentin in the presence of several units of λPPase corresponding to Fig. 6b lanes 3-12 (d). Data were collected from four independent experiments (c and d). Error bars indicate ± SD of four experiments (c and d)