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. 2018 May 1;34(5):459–466. doi: 10.1089/aid.2017.0118

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Copyright 2018, Mary Ann Liebert, Inc.

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FIG. 1. — No alteration of histology and viability of colonic tissue exposed to fresh or frozen-then-thawed WS and SP. (a) Immersion of freshly acquired colonic tissue for 2 h in various concentrations of fresh or frozen-then-thawed WS (10%, 25%, 50%, 100%) and SP (10%, 100%) did not significantly induce colonic tissue histology changes ex vivo as assessed by a single-blinded pathologist. All of the tissues in 100% semen concentration scored 1–2, mean event rate 2 (0). The tissues in 10% WS or SP scored 1–3, mean event rate of 1.78 (0.67). Tissue in control condition without semen or SP scored 1–3, mean event rate 1.9 (0.73). (b) The effect of semen and SP on tissue viability in the MTT assay was defined as >40% compared to the experimental media controls. No reduction of tissue viability below 40% was noted after 2 h exposure to frozen-then-thawed WS and SP (10%, 50%, 100%) nor fresh WS or SP (50%). SP, seminal plasma; WS, whole semen.