FIG. 2.

BM-MSCs are reduced in vivo after IFN-γ exposure. (A) Flow cytometric analysis and (B) quantification of IFN-γ production by BM T cells and NK cells from ARE-Del mice and WT controls. CD4+ and CD8+ T cells were defined as CD3⁺CD4⁺CD8⁻ and CD3⁺CD4⁻CD8⁺, respectively. CM = central memory (CD44⁺CD62L⁺). EM = effector memory (CD44⁺CD62 L⁻). NK cells were defined as CD3⁻CD56⁺ and NKT cells were CD3⁺CD56⁺. Cells were incubated for 4 h without any stimulus in the presence of Brefeldin A (mean–SD; n = 2–3). (C) Absolute number of total BM cells and (E) relative numbers of BM stromal cell subsets and BM-MSCs in ARE-Del mice and WT controls (mean–SEM; n = 5). (D) Gating strategy for murine BM MSCs (F) CXCL12 and SCF expression by sorted BM-MSC, normalized to Cyclophilin (mean–SEM; n = 5). (G) Percentage of BM-MSCs in total BM cells of ARE-Del mice and WT controls between 3–30 weeks of age (mean–SEM; n = 3–5). *P < 0.05; ***P < 0.001 unpaired t-test. BM, bone marrow; WT, wild type; SEM, standard error of the mean; ARE, AU-rich elements.