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. 2018 May 1;27(9):579–589. doi: 10.1089/scd.2017.0196

FIG. 3.

FIG. 3.

HS(P)C numbers and quiescence in ARE-Del mice. (A) Gating strategy for SLAM HSC. (B) Percentage of LSK and (C) absolute number of HS(P)C in ARE-Del mice and WT controls (mean–SEM; n = 5). (D) Flow cytometric analysis and (E) quantification of Ki-67-negative HS(P)C in ARE-Del mice and WT controls (mean–SEM; n = 5) (F) LT-HSC were sorted and cultured for 7 days in X-vivo medium with TPO, SCF, IL-3, IL-6, and Flt3-L. After 7 days, flow cytometric analysis was performed to identify phenotypic HS(P)Cs (mean–SEM; n = 7–8). (G) Absolute numbers of BM-MSCs and (H) LT-HSC in 1-year-old ARE-Del mice and WT controls. *P < 0.05; **P < 0.01, ***P < 0.001 unpaired t-test. HSC, hematopoietic stem cells; LT-HSC, long-term HSC; TPO, thrombopoietin; LSK, Lineage− Sca-1+ c-kit+.