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. 2018 May 4;475(9):1553–1567. doi: 10.1042/BCJ20170923

Figure 6. Mutation of a predicted translocation cavity in ABCG2.

Figure 6.

(A and B) An additional set of residues (green) were mutated to alanine based upon their localisation to the proposed translocation cavity between the surface and buried MX binding sites. (C and D) Mutants were sfGFP-tagged and expressed in HEK293T cells, with all bar L633A forming mature protein at the plasma membrane. (E) Functional analysis of the mutants demonstrated an enhanced transport of DNR in M548A, and altered MX transport for M541A and F571A and M636A. All data result from at least three independent experiments with error bars denoting standard error of the mean.