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. Author manuscript; available in PMC: 2019 Jan 9.

Published in final edited form as: Biochemistry. 2017 Oct 31;57(1):117–135. doi: 10.1021/acs.biochem.7b00838

Table 1.

Apparent Michaelis-Menten parameters for E. coli, S. aureus, and human GS.

GS	ATP			L-Glu
GS	K_m (mM)	k_cat (min⁻¹)	k_cat/K_m (min⁻¹mM⁻¹)	K_m (mM)	k_cat (min⁻¹)	k_cat/K_m (min⁻¹mM⁻¹)
E. coli	0.7 ± 0.1^a	7300 ± 300	10400 ± 1500	7.2 ± 0.8	7900 ± 300	1100 ± 100
S. aureus	1.8 ± 0.1	720 ± 20	400 ± 30	52 ± 7	1400 ± 100	27 ± 4
Homo sapien	0.7 ± 0.1	2600 ± 100	3700 ± 500	37 ± 5	5100 ± 300	140 ± 20

^a

All standard deviations were determined for three independent trials.