Analyses gastrocnemius muscle morphology and wet weight for evaluation of
post-injury muscle atrophy. A, Representative photographs of gastrocnemius
muscles from both hind limbs in each group. Scale bar, 25mm; B, Representative
light microscopy images of cross-sectioned gastrocnemius muscles following
H&E staining 30 days post-injury. Scale bar, 50μm; C. Histograms
showing the percentage of cross-sectional area of muscle fibers quantified with
Image-Pro Plus software analysis of light microscopy results; D. Wet weight
ratios of gastrocnemius muscles in each group at 30 days post injury. Free GFs
vs PNI-diabetes: **P <0.01,
GFs-HP hydrogel vs PNI-diabetes:
###P <0.001, GFs-HP
hydrogel vs Free GFs: &P <0.05. All data
represent mean values±SEM, n=5 for each group.