Histological and microstructure evaluation of injured sciatic nerve. A.
longitudinal sections of regenerated nerve stained with Masson’s
trichrome 30 days after injury, Scale bar=50μm; B. TEM images of
cross-sections of lesion regions, Scale bar=2μm; C. Double
immunofluorescence staining for NF-200 and MBP-positive cells of the
longitudinal sections in each experimental group. Scale
bar=25μm; D, E. Quantification analysis of myelinated axonal
count and G-ratio (G-ratio=axon diameter/fiber diameter) in the
indicated groups; F, G. Quantitative analyses of fluorescence intensity of
pixels for MBP and NF-200. Values are expressed as mean±SEM, n=5
per group. Free GFs vs PNI-diabetes: *P
<0.05, **P <0.01, GFs-HP
hydrogel vs PNI-diabetes:
###P <0.001, GFs-HP
hydrogel vs Free GFs: &P <0.05,
&&P <0.01.