GFs-HP hydrogel facilitates SCs proliferation. A. The double immunofluorescence
staining of Ki67 (green) with the GFAP (red) to label proliferating SCs in
longitudinal sciatic nerve sections, Scale bar=50μm; B. The
percentages of cells double-positive for Ki67 and GFAP out of total DAPI
positive cells (representing proliferation rate of SCs); C. The protein levels
of Ki67 and PCNA at 30 days after injury by western blotting; D, E.
Densitometric analyses of PCNA and Ki67, respectively. GAPDH was used for band
density normalization. Data presented as mean±SEM, n=5 for each
group. Free GFs vs PNI-diabetes: **P
<0.01, ***P <0.001,
GFs-HP hydrogel vs PNI-diabetes:
###P<0.001, GFs-HP hydrogel vs Free GFs:
&P <0.05,
&&P <0.01. (For interpretation of the
references to color in this figure legend, the reader is referred to the Web
version of this article.)