Fig. 4.

a) Cell viability of MC3T3-E1 pre-osteoblasts cultured. The absorbance was detected at wavelength 490 nm and 570 nm b) ALP activities of MCM3T3-E1 pre-osteoblasts cultured after 3,7 and 14 days. The absorbance was detected at the wavelength of 520 nm c) Microscopic view of MCM3T3-E1 cultured on the samples a)Ti and b) 0 PLGA layer c) 3 PLGA layers after 8 h.