Fig. 3. Confocal images of living HeLa cells after incubation with TTVP (500 nM) for (a) 10 min by the use of a washing procedure after incubation, (b) 10 min, (c) 5 min, (d) 2 min, (e) 30 s, and (f) an extremely short incubation period (around 3 s) by the use of the wash-free procedure after incubation. λex: 488 nm (1% laser power). (g) Schematic illustration of plasma membrane-specific imaging with TTVP. Scale bar = 20 μm. (h) Fluorescence spectrum of the plasma membrane of living HeLa cells stained with TTVP, and the fluorescence spectrum of TTVP nanoaggregation in the THF/H2O mixture.
