FIGURE 7:

Membrane fluidity is increased in the lem3Δ sfk1Δ double mutant. (A) TMA-DPH is retained in the plasma membrane. Cells were cultured in SD medium at 25°C and treated with 0.5 μM TMA-DPH. Stained cell suspensions were kept on ice for 45 min and subjected to fluorescence microscopy. Scale bar: 5 μm. (B) Steady-state anisotropy is decreased in the lem3Δ sfk1Δ mutant. Cells were cultured in SD medium at 25°C, treated with 0.5 μM TMA-DPH, and subjected to an analysis by spectrophotometry. Measurement of anisotropy was performed as described under Materials and Methods. Average of steady-state anisotropy is presented, with SD from three independent experiments (*p < 0.05; Tukey’s test).