Skip to main content
. 2018 May 15;29(10):1203–1218. doi: 10.1091/mbc.E17-04-0217

FIGURE 8:

FIGURE 8:

Ergosterol is decreased in the lem3Δ sfk1Δ mutant. (A) Total phospholipid composition was not altered by either deletion or overexpression of SFK1. Cells were grown in YPDA or SDA-U medium, followed by extraction of total cellular lipids. Phospholipid contents were analyzed by TLC as described under Materials and Methods. The data present percentages of total phospholipids with SD of three independent experiments. (B) Ergosterol is decreased in lem3Δ sfk1Δ cells but not altered in SFK1-overexpressing cells. Cells were grown in YPDA or SDA-U medium, followed by extraction of total cellular lipids. Ergosterol contents were analyzed by TLC as described under Materials and Methods. Percentage average ergosterol levels relative to wild-type or wild-type carrying YEp24 vector are presented, with SD of six independent experiments (*p < 0.05, **p < 0.01; Tukey’s test). Top panel shows the scanned images of HPTLC plates.