Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 15;29(10):1258–1269. doi: 10.1091/mbc.E17-12-0714

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Zuzow et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

PMC Copyright notice

FIGURE 2: — Identification of Ltn1-interacting proteins using proximity labeling approaches. (A) 293 Flp-In cells with dox-induced expression of FLAG-HA(F)-, BirA*-FLAG(B)-, or FLAG-APEX2(A)–tagged GFP or Ltn1 were treated with doxycycline overnight, and protein biotinylation was induced as described for BirA*- or APEX2-tagged cell lines. Whole-cell extracts were immunoblotted with a-FLAG antibodies or probed with SA-HRP. Short (s) or long (l) exposures are depicted. (B) The summed total spectral counts (TSCs) from replicate experiments corresponding to NEMF (left) or VCP (right) from streptavidin or anti-FLAG–isolated and identified proteins from FLAG-HA(F)-, BirA*-FLAG(B)-, or FLAG-APEX2(A)–tagged GFP- or Ltn1-expressing cell extracts. For panels B–D, all IPs were performed in triplicate from distinct experiments and cell lysates. (C) The number of high-confidence interacting proteins (HCIPs) for Ltn1 for each of the three proteomic platforms was determined using a modified CompPASS scoring method. (D) The normalized interaction scores as determined by the CompPASS scoring method for top-scoring Ltn1 interacting proteins identified using the BioID approach is shown.