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. 2018 Feb 27;41(4):351–361. doi: 10.14348/molcells.2018.2195

Fig. 4. Anthocyanin and flavonol accumulation in Col-0, atmyb56 and AtMyb56 OE seedlings.

Fig. 4

(A) Four-day-old seedlings of Col-0, atmyb56#1, and atmyb56#2 exposed to 0, 100 or 200 mM sucrose for 6 h were used for anthocyanin extraction. The anthocyanin content is shown, combined from two independent experiments with three replicates each. (B) Four-day-old seedlings of Col-0, atmyb56#1, and AtMyb56 OE#2 were used for anthocyanin extraction. The anthocyanin content is shown, combined from two independent experiments with three replicates each. (C) Roots of four-day-old Col-0, atmyb56#1, atmyb56#2, and AtMyb56 OE#2 seedlings were used for flavonol-DPBA staining to detect flavonol accumulation. Quercetin-DPBA conjugates display a yellow fluorescent color while kaempferol-DPBA conjugates show green fluorescence. Error bars represent the standard errors (SEs) of three biological replicates. Asterisks indicate significant differences from the Col-0 plants (P < 0.05).