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. 2018 Apr 5;41(4):320–330. doi: 10.14348/molcells.2018.2292

Fig. 4. δ-Catenin and c-Cbl compete for EGFR binding.

Fig. 4

(A) Immunoprecipitation with anti-EGFR antibody followed by immunoblotting with anti- δ-catenin antibody and anti-c-Cbl antibody of lysate obtained from transfection of respective plasmid in CWR22Rv-1 cells that were serum starved followed by 2 min 100 ng/ml EGF treatment. This experiment was performed three independent times (B) CWR22Rv-1 cells were transfected with EGFR-GFP, c-Cbl, and δ-catenin (one, two, and three times) and serum starved 24 h followed by 100 ng/ml EGF treatment for 2 min. Lysates were immunoprecipitated with an anti-EGFR antibody followed by immunoblotting with anti-c-Cbl and anti-EGFR antibodies. The data represents three independent experiments. (C) Quantitation of the data shown in Fig. 4B. Data shown represents mean ± SD. All p values were determined with unpaired Student’s t test: *p < 0.05, **p < 0.001 and ***p < 0.0001.