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. Author manuscript; available in PMC: 2018 May 4.
Published in final edited form as: Mech Ageing Dev. 2016 Jan 4;158:62–69. doi: 10.1016/j.mad.2015.12.005

Fig. 5.

Fig. 5

(A) Effects of sirtinol on hCMV-induced expression of Rb, pRb Ser780, p16INK4, and p53 in WI-38 fibroblasts at 72 hours post infection (hpi). Serum starved low passage WI-38 cells (PD30) were treated by sirtinol at indicated concentration added 2h before hCMV inoculation (MOI 0.01) (−2h) and then were harvested at 72 hpi for expression of the above molecules using Western blot analysis. Beta-actin expression level is shown as loading control. Representative images were acquired from three different experiments. (B) Quantitative analysis of the protein levels of Rb, pRbSer780, p16INK4, and p53. Bars represent relative protein levels counted as D1/D0 (the value for Mock was set as 1.0), where D0 and D1 stand for the optical density of beta-actin ladder and sample ladder, respectively. The optical density for each ladder was calculated by Image J soft ware. Data were obtained from three independent experiments. *P < 0.05 versus Mock group; **P < 0.01 versus Mock group; #P < 0.05 versus hCMV alone group; ##P < 0.01 versus hCMV alone group.