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CD38 E230Q PCR Assays
| Assay | BAC Region | Primer Sequences | Product Size | |
|---|---|---|---|---|
| BAC | WT | |||
| A | T7 end | CGGCCGCTAATACGACTCACTATAGGGAGA TTTATTTGAGACGGCTACGAAGGCTG |
362 bp | - |
| B | CD157 Insertion | AGTTCTAGAGCGGCCAGGGAGTTGCTTCCGAATGTATAGCC TCCACACATGCTCCACACTCGCTC |
553 bp | 385 bp |
| C | CD38 Insertion | CCAAGCTATCGAATTCGAAACTGAGATAAAGTGTGA ACAGTGCTGCCTAAAGACCTGCACACTTCCAAAGGTGCTGAA |
856 bp | 751 bp |
| D | Sp6 end | CCCTTTCAGGCACCCAGTTCTC CGCCTGGCCGTCGACATTTA |
428 bp | - |
