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Biochemical assay employed for the estimation of enzymatic activity of BioA. KAPA, SAM and PLP in the presence of BioA react to form DAPA. DAPA in the presence of derivatizing solution composed of OPA–2ME reagent gives a fluorescent adduct, which is then measured at an emission wavelength of 470 nm with an excitation wavelength of 410 nm.
Abbreviations: BioA, 7,8-diaminopelargonic acid synthase; SAM, S-adenosylmethionine; PLP, pyridoxal-5′-phosphate; OPA, orthophthaldehyde.
