Table III.
Investigation of the motif responsible for protein-to-protein interaction between OsMADS6 and OsMADS14
| Activation Domain Plasmid | Binding Domain Plasmid | β-Galactosidase Activitya |
|---|---|---|
| pAD/OsMADS14-MIKC14 | pBD/OsMADS6-MIKC14 | 10.21 ± 1.007 |
| pAD/OsMADS14-MIKC14 | pBD/OsMADS6-MI | 0.09 ± 0.011 |
| pAD/OsMADS14-MI | pBD/OsMADS6-MIKC14 | 0.06 ± 0.007 |
| pAD/OsMADS14-MI | pBD/OsMADS6-MI | 0.07 ± 0.032 |
| pAD/OsMADS14-KC | pBD/OsMADS6-K | 13.22 ± 1.310 |
| pAD/OsMADS14-KC | pBD/OsMADS6-KC14 | 62.12 ± 7.583 |
| pAD/OsMADS14-KC | pBD/OsMADS6-KI | 0.14 ± 0.015 |
| pAD/OsMADS14-KC | pBD/OsMADS6-KII | 18.21 ± 3.224 |
| pAD/OsMADS14-KC | pBD/OsMADS6-KIII | 0.16 ± 0.023 |
| pAD/OsMADS14-KC | pBD/OsMADS6-KII+KIII | 53.40 ± 2.824 |
| pAD/OsMADS6-MIKC14 | pBD/OsMADS14-MIKC14 | 8.14 ± 0.075 |
| pAD/OsMADS6-MI | pBD/OsMADS14-MIKC14 | 0.08 ± 0.010 |
| pAD/OsMADS6-MIKC14 | pBD/OsMADS14-MI | 0.06 ± 0.011 |
| pAD/OsMADS6-MI | pBD/OsMADS14-MI | 0.05 ± 0.008 |
| pAD/OsMADS6-K | pBD/OsMADS14-KC14 | 3.21 ± 1.024 |
| pAD/OsMADS6-KC14 | pBD/OsMADS14-KC14 | 61.23 ± 7.972 |
| pAD/OsMADS6-KC | pBD/OsMADS14-KC14 | 59.14 ± 4.927 |
| pAD/OsMADS6-KI | pBD/OsMADS14-KC14 | 0.04 ± 0.007 |
| pAD/OsMADS6-KII | pBD/OsMADS14-KC14 | 0.06 ± 0.032 |
| pAD/OsMADS6-KIII | pBD/OsMADS14-KC14 | 0.06 ± 0.016 |
| pAD/OsMADS6-KII+KIII | pBD/OsMADS14-KC14 | 17.43 ± 2.972 |
a
β-Galactosidase activity unit = 1,000 × A420/[A600 × reaction time (min) × volume of culture (mL)].