Table 1. Oligonucleotide primers.
Primers | Direction | Nucleotide sequence from 5' to 3' |
---|---|---|
A1 | forward | GGGGTACCCCCGATGAGAGCAGCTGGTATTG |
B1 | reverse | CAGAATCATTTGTTTCATCAAACAATGCGCCATCATAGTTT |
C1 | forward | TGGAGCAGGTTGAGAAGGAAGGTTCTGCGCGAGTGATAGAT |
D1 | reverse | CAACAAGCTTCAAAGCATCGTTGG |
E1 | forward | TTGATGAAACAAATGATTCTGATG |
F1 | reverse | TTCCTTCTCAACCTGCTCCA |
D2 | reverse | CAACAGGATCCAAAGCATCGTTGG |
B2 | forward | TGAGTGAACCACAGCCAGAA |
B3 | forward | TCAGCAACGTGTGTCTTGGT |
B4 | reverse | CGAACCTTTACTTCGGCATC |
B5 | reverse | GTGATTCCCTTTGCTCTGC |
The bold sections in the nucleotide sequences indicate the sites of restriction endonucleases used to create the design.