Table 1. Summarizes the proteins of watery saliva of S. furcifera identified by LC-MS/MS.
| Protein identification | No. of unique peptidesa | Protein domainb | THMHHc | TargetPd | SignalPe | Function group |
|---|---|---|---|---|---|---|
| Cytochrome c oxidase subunit 1 | 22 | Cytochrome C and Quinol oxidase polypeptide I | 12 | S | Yes | Oxidoreductases |
| NADH-ubiquinone oxidoreductase chain 6 | 2 | plastoquinone oxidoreductase chain 6 | 4 | S | Yes | Oxidoreductases |
| NADH-ubiquinone oxidoreductase chain 3 | 3 | NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 | 3 | S | Yes | Oxidoreductases |
| NADH-ubiquinone oxidoreductase chain 2 | 1 | Proton-conducting membrane transporter | 5 | S | Yes | Oxidoreductases |
| Cytochrome P450 CYP419A1 | 1 | Cytochrome P450 | 0 | / | No | Oxidoreductases |
| Cytochrome b | 3 | Cytochromeb (C-terminal)/b6/petD Cytochrome b/b6/petB; Cytochrome b(N-terminal)/b6/petB | 4 | S | No | Oxidoreductases |
| Cytochrome c oxidase subunit 2 | 1 | Cytochrome C oxidase subunit II, periplasmic domain; Cytochrome C oxidase subunit II, transmembrane domain | 2 | S | No | Oxidoreductases |
| NADH dehydrogenase subunit 6 | 1 | / | 4 | S | Yes | Oxidoreductases |
| Peptidylglycine α-hydroxylating monooxygenase | 1 | Copper type II ascorbatedependent monooxygenase, C-terminal domain; Copper type II ascorbate-dependent monooxygenase, N-terminal domain | 1 | S | Yes | Oxidoreductases |
| Inosine-5'-monophosphate dehydrogenase | 1 | IMP dehydrogenase / GMP reductase domain; CBS domain | 0 | / | No | Oxidoreductases |
| Maestro heat-like repeat-containing protein family member 1 | 1 | / | 0 | S | No | Oxidoreductases |
| Aldo-keto reductase | 1 | Aldo/keto reductase family | 0 | / | No | Oxidoreductases |
| Uncharacterized protein LOC100165697 | 1 | / | 0 | / | No | Oxidoreductases |
| Isolate 9 chitinase | 1 | Glycosyl hydrolases family 18 | 0 | M | No | Hydrolases |
| Carboxylesterase | 2 | Carboxylesterase family | 0 | S | Yes | Hydrolases |
| Non-lysosomal glucosylceramidase | 1 | Glycosyl-hydrolase family 116, catalytic region; beta-glucosidase 2, glycosyl-hydrolase family 116 N-term | 1 | / | No | Hydrolases |
| Dicer 2 | 1 | PAZ domain; Ribonuclease III domain | 0 | / | No | Hydrolases |
| Ankyrin repeat domain-containing protein | 1 | Ankyrin repeats (3 copies); Ankyrin repeats (many copies) | 0 | / | No | Hydrolases |
| Uncharacterized protein LOC105391939 isoform X3 | 1 | Phospholipase A2 | 1 | / | No | Hydrolases |
| Hypothetical protein D910_09660 | 3 | Endoribonuclease XendoU | 0 | S | No | Hydrolases |
| Uncharacterized protein LOC100164352 | 1 | / | 0 | / | No | Hydrolases |
| Hypothetical protein ANCCEY_11605 | 1 | / | 0 | / | No | Hydrolases |
| Placental protein 11 | 1 | Endoribonuclease XendoU | 1 | M | No | Hydrolases |
| Plancitoxin-1 isoform X1 | 2 | Deoxyribonuclease II | 1 | S | No | Hydrolases |
| GA19137 isoform A | 1 | USP8 dimerisation domain; Rhodanese-like domain; Ubiquitin carboxyl-terminal hydrolase | 0 | / | No | Hydrolases |
| N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase | 1 | GlcNAc-PI de-N-acetylase | 1 | S | Yes | Hydrolases |
| Ubiquitin carboxyl-terminal hydrolase 7 | 1 | MATH domain; Ubiquitin carboxyl-terminal hydrolase; ICP0-binding domain of Ubiquitin-specific protease 7; Ubiquitin-specific protease C-terminal | 0 | / | No | Hydrolases |
| Uncharacterized family 31 glucosidase KIAA1161 isoform X1 | 1 | Glycosyl hydrolases family 31 | 0 | / | No | Hydrolases |
| Constitutive coactivator of peroxisome proliferator-activated receptor | 1 | / | 0 | / | No | Hydrolases |
| Endonuclease-reverse transcriptase | 1 | / | 0 | / | No | Hydrolases |
| Neprilysin-11-like isoform X4 | 1 | Peptidase family M13 | 1 | S | No | Peptidases |
| Xaa-Pro dipeptidase | 1 | FAST kinase-like protein, subdomain 1 | 0 | / | No | Peptidases |
| Lon protease-like protein | 1 | ATP-dependent protease La (LON) substrate-binding domain; ATPase family associated with various cellular activities (AAA); Lon protease (S16) C-terminal proteolytic domain | 0 | M | No | Peptidases |
| Serine protease 6 | 1 | Trypsin | 1 | S | Yes | Proteases |
| Stubble-2 | 1 | Trypsin | 0 | / | No | Proteases |
| Activated CDC42 kinase 1 | 1 | GTPase binding; Protein tyrosine kinase | 0 | / | No | Transferases |
| ATP citrate lyase isoform X1 | 1 | ATP-grasp domain; ATP citrate lyase citrate-binding; CoA binding domain; CoA-ligase; Citrate synthase, C-terminal domain | 0 | / | No | Transferases |
| Reverse transcriptase | 3 | Reverse transcriptase (RNA-dependent DNA polymerase) | 0 | / | No | Transferases |
| Hypothetical protein D910_10443 | 1 | Reverse transcriptase (RNA-dependent DNA polymerase) | 0 | / | No | Transferases |
| Uncharacterized protein LOC105556507 | 1 | / | 0 | / | No | Transferases |
| UDP-glucuronosyltransferase 2C1 | 1 | UDP-glucoronosyl and UDP-glucosyl transferase | 1 | / | No | Transferases |
| Histone-lysine N-methyltransferase SETMAR | 1 | / | 0 | / | No | Transferases |
| Protein purity of essence isoform X4 | 1 | E3 ubiquitin-protein ligase UBR4 | 0 | / | No | Transferases |
| RNA-directed DNA polymerase from mobile element jockey-like | 1 | / | 0 | / | No | Transferases |
| Hypothetical protein L798_09082 | 1 | Ring finger domain;CUE domain | 0 | / | No | Transferases |
| FAST kinase domain-containing protein 1 | 1 | FAST kinase-like protein, subdomain 2 | 0 | / | No | Transferases |
| Carbonic anhydrase 2 | 1 | Eukaryotic-type carbonic anhydrase | 1 | S | Yes | Lyases |
| Cytoplasmic aconitate hydratase-like | 1 | Aconitase family (aconitate hydratase); Aconitase C-terminal domain | 0 | / | No | Lyases |
| Ornithine decarboxylase | 1 | Pyridoxal-dependent decarboxylase, pyridoxal binding domain; Pyridoxal-dependent decarboxylase, C-terminal sheet domain | 0 | / | No | Lyases |
| Sphingosine-1-phosphate lyase | 1 | Pyridoxal-dependent decarboxylase conserved domain | 0 | / | No | Lyases |
| Protein disulfide-isomerase | 1 | Thioredoxin | 0 | S | Yes | Isomerases |
| ATP-binding domain-containing protein 4 | 1 | Diphthamide synthase; Endoribonuclease L-PSP | 0 | / | No | Ligases |
| CTP synthase | 1 | CTP synthase N-terminus; Glutamine amidotransferase class-I | 1 | / | No | Ligases |
| ATP synthase F0 subunit 8 | 3 | / | 1 | S | Yes | ATP synthases |
| ATP synthase subunit a | 1 | ATP synthase A chain | 5 | S | No | ATP synthases |
| ATP synthase protein 8 | 1 | ATP synthase protein 8 | 1 | S | Yes | ATP synthases |
| Vacuolar ATP synthase subunit E | 1 | ATP synthase (E/31 kDa) subunit | 0 | / | Yes | ATP synthases |
| ATP synthase-coupling factor 6 | 1 | Mitochondrial ATP synthase coupling factor 6 | 0 | M | No | ATP synthases |
| Uncharacterized protein LOC100632426 | 1 | Iron-sulphur cluster biosynthesis | 0 | / | No | ATP synthases |
| ATP-binding cassette sub-family G member 4-like protein | 3 | ABC-2 type transporter; ABC transporter | 7 | / | No | ABC transporter |
| ATP-binding cassette sub-family D member 2-like protein | 1 | ABC transporter transmembrane region 2; ABC transporter | 4 | / | No | ABC transporter |
| ATP-binding cassette sub-family B member 7 | 1 | ABC transporter transmembrane region | 0 | M | No | ABC transporter |
| ABC protein subfamily ABCH | 1 | ABC transporter; ABC-2 family transporter protein | 5 | / | No | ABC transporter |
| Multidrug resistance-associated protein 1 isoform X1 | 1 | ABC transporter transmembrane region; ABC transporter | 16 | S | Yes | ABC transporter |
| Sly1-like protein | 1 | Sec1 family | 0 | / | No | Endoplasmic reticulum (ER)–Glogi transporter |
| Uncharacterized protein LOC100165575 | 1 | / | 1 | S | No | Ion transporter |
| Piezo-type mechanosensitive ion channel component 2 isoform X7 | 1 | Piezo non-specific cation channel, R-Ras-binding domain; Piezo | 31 | S | Yes | Ion transporter |
| F-box/LRR-repeat protein 7 | 1 | F-box-like; Methyl-CpG binding domain | 0 | / | No | Ion transporter |
| Organic cation transporter protein isoform X2 | 1 | Sugar (and other) transporter | 10 | S | Yes | Ion transporter |
| Uncharacterized protein LOC105568157 | 1 | / | 0 | / | No | Ion transporter |
| Golgin subfamily A member 4 | 1 | GRIP domain | 0 | / | No | Golgi transporter |
| Apolipophorin | 1 | von Willebrand factor type D domain | 0 | / | No | Lipid transporter |
| Lipophorin precursor | 1 | Lipoprotein amino terminal region; Domain of unknown function (DUF1943); Domain of Unknown Function (DUF1081); von Willebrand factor type D domain | 0 | S | Yes | Lipid transporter |
| Vitellogenin | 1 | Lipoprotein amino terminal region; Domain of unknown function (DUF1943); von Willebrand factor type D domain | 0 | S | Yes | Lipid transporter |
| Uncharacterized protein LOC100119722 | 1 | Saposin A-type domain; Saposin-like type B, region 1; Saposin-like type B, region 2 | 2 | S | Yes | Lysosome transporter |
| Vam6/Vps39-like protein | 1 | CNH domain; Vacuolar sorting protein 39 domain 1; Vacuolar sorting protein 39 domain 2 | 0 | / | No | Lysosome transporter |
| Vacuolar protein sorting-associated protein 28-like protein | 1 | VPS28 protein | 0 | / | No | Lysosome transporter |
| Run and tbc1 domain-containing protein | 1 | Rab-GTPase-TBC domain | 0 | / | No | Lysosome transporter |
| Hypothetical protein L798_05106 | 1 | Thioredoxin | 2 | S | Yes | Protein transporter |
| Charged multivesicular body protein 3 | 1 | Snf7 | 0 | / | No | Vacuolar transporter |
| Calexcitin-1 isoform X1 | 1 | EF hand; Deoxyribonuclease II | 0 | S | No; | Calcium ion binding protein |
| Hypothetical protein TcasGA2_TC004855 | 1 | LETM1-like protein | 1 | M | No | Calcium ion binding protein |
| EF-hand domain containing protein | 1 | EF hand | 0 | / | No | Calcium ion binding protein |
| Mucin-like protein | 11 | / | 1 | S | Yes | Sheath proteins |
| Actin | 2 | Actin | 0 | / | No | Cytoskeleton protein |
| Myosin-IIIa | 1 | IQ calmodulin-binding motif; Myosin head (motor domain) | 0 | / | No | Cytoskeleton protein |
| Kinesin light chain | 1 | Tetratricopeptide repeat | 0 | / | No | Cytoskeleton protein |
| Microtubule-actin cross-linking factor 1 isoform X4 | 1 | Spectrin repeat; EF-hand domain pair; Growth-Arrest-Specific Protein 2 Domain | 0 | / | No | Cytoskeleton proteins |
| Kinesin-like protein KIF21A isoform X2 | 1 | Kinesin motor domain; WD domain, G-beta repeat | 0 | / | No | Cytoskeleton proteins |
| Cordon-bleu protein-like 1 | 1 | / | 0 | / | No | Cytoskeleton protein |
| Zinc finger protein 239-like | 1 | Zinc finger, C2H2 type; C2H2-type zinc finger | 0 | / | No | Zinc finger protein |
| Zinc finger matrin-type protein | 1 | / | 0 | / | No | Zinc finger protein |
| Apterous a | 1 | LIM domain; Homeobox domain | 0 | / | No | Transcription factors |
| Ecdysteroid receptor | 1 | Ligand-binding domain of nuclear hormone receptor; Zinc finger, C4 type (two domains) | 0 | / | No | Transcription factors |
| Transcriptional regulator ATRX-like | 1 | SNF2 family N-terminal domain; Helicase conserved C-terminal domain | 0 | / | No | Transcription factors |
| RNA polymerase-associated protein CTR9-like protein | 1 | Tetratricopeptide repeat | 0 | / | No | Transcription factors |
| Conserved hypothetical protein | 1 | Leo1-like protein | 0 | / | No | Transcription factors |
| Transcription initiation factor IIA subunit 1 | 1 | Transcription factor IIA, alpha/beta subunit | 0 | M | No | Transcription factors |
| Hypothetical protein L798_07165 | 1 | MH1 domain | 0 | / | No | Transcription factors |
| Hypothetical protein L798_07469 | 1 | / | 0 | M | No | Transcription factors |
| Elongation factor Tu | 1 | Elongation factor Tu GTP binding domain; Elongation factor Tu domain 2; Elongation factor Tu C-terminal domain | 0 | / | No | Translation factors |
| Elongation factor G2 | 1 | Elongation factor Tu; GTP binding domain | 0 | M | No | Translation factors |
| Cryptochrome 2 | 1 | DNA photolyase; FAD binding domain of DNA photolyase | 0 | / | No | DNA repair and recombination protein |
| Thyroid receptor-interacting protein 11 | 1 | GRIP domain | 0 | / | No | DNA repair |
| Uncharacterized protein LOC103516581 | 1 | / | 0 | M | No | DNA binding |
| Cold shock domain-containing protein E1 | 1 | 'Cold-shock' DNA-binding domain | 0 | / | No | DNA binding |
| HMG box-containing protein 1 | 1 | / | 0 | / | No | DNA binding |
| AT-rich interactive domain-containing protein 4B | 1 | RBB1NT (NUC162) domain; ARID/BRIGHT DNA binding domain | 0 | / | No | DNA binding |
| Nucleoprotein TPR | 1 | / | 0 | / | No | DNA binding |
| La-related protein 1B | 1 | / | 0 | / | No | mRNA biosynthesis |
| Mitochondrial fission protein | 1 | Fis1 C-terminal tetratricopeptide repeat; Fis1 N-terminal tetratricopeptide repeat | 1 | S | No | Mitochondrial biogenesis |
| ESF1 homolog | 1 | NUC153 domain | 0 | / | No | Nucleotide binding |
| Bromodomain-containing protein 7 | 1 | Bromodomain; Domain of unknown function (DUF3512) | 0 | / | No | Protein binding |
| Bromodomain and WD repeat-containing protein 2 | 1 | / | 0 | / | No | Protein binding |
| Chaoptin | 1 | / | 6 | M | No | Protein binding |
| Kazrin-A | 1 | SAM domain (Sterile alpha motif) | 0 | / | No | Protein binding |
| Sorting nexin-17 | 1 | / | 0 | / | No | Protein binding |
| Ring finger protein 160 | 1 | Ring finger domain | 0 | / | No | Ring finger protein |
| Cisplatin resistance-associated overexpressed protein | 1 | LUC7 N_terminus | 0 | M | No | RNA binding |
| Teneurin-3-like | 1 | / | 0 | M | No | RNA binding |
| R3H domain-containing protein 1 isoform X1 | 1 | R3H domain; SUZ domain | 0 | / | No | RNA binding |
| Coiled-coil domain-containing protein 12 | 1 | cwf18 pre-mRNA splicing factor | 0 | / | No | Spliceosome |
| Transcription elongation regulator 1 | 1 | WW domain | 0 | / | No | Spliceosome |
| Integrator complex subunit 1 isoform X1 | 1 | / | 0 | / | No | Spliceosome |
| Anaphase-promoting complex subunit | 1 | / | 0 | / | No | Ubiquitin |
| CD109 antigen-like | 1 | MG2 domain; Alpha-2-macroglobulin family N-terminal region; Alpha-2-macroglobulin family; Alpha-macro-globulin thiol-ester bond-forming region; A-macroglobulin complement component; A-macroglobulin receptor | 0 | S | Yes | Antigen |
| Tubulin-specific chaperone C | 1 | Tubulin-specific chaperone C N-terminal domain; Tubulin binding cofactor C | 0 | / | No | Chaperone protein |
| Interaptin isoform X2 | 1 | / | 1 | S | Yes | Ribosome-binding protein |
| Notchless protein homolog 1-like | 1 | NLE (NUC135) domain; WD domain, G-beta repeat | 0 | / | No | Ribosome biogenesis |
| mRNA turnover protein 4 homolog | 1 | Ribosomal protein L10 | 0 | / | No | Ribosome biogenesis |
| Hypothetical protein KGM_12879 | 1 | RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) | 0 | / | No | Ribosome biogenesis |
| Putative RNA-binding protein 19 | 1 | RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) | 0 | / | No | Ribosome biogenesis |
| Nucleolar complex protein 3-like protein | 1 | Nucleolar complex-associated protein; CBF/Mak21 family | 0 | / | No | Ribosome biogenesis |
| 30S ribosomal protein S2 | 1 | Ribosomal protein S2 | 0 | / | No | Ribosomal protein |
| 39S ribosomal protein L38 | 1 | Phosphatidylethanolamine-binding protein | 0 | / | No | Ribosomal protein |
| Uncharacterized protein LOC105558474 | 1 | Ribosomal protein S12/S23 | 0 | / | No | Ribosomal protein |
| Heat shock cognate 70 protein | 1 | Hsp70 protein | 0 | / | No | Heat shock protein |
| DEP domain-containing protein 5 isoform X4 | 1 | / | 0 | / | No | Signal transduction protein |
| Mothers against decapentaplegic homolog 3 | 1 | MH2 domain | 0 | M | No | Signal transduction protein |
| Protein numb | 1 | Phosphotyrosine interaction domain (PTB/PID) | 0 | / | No | Signal transduction protein |
| Protein numb-like | 1 | NUMB domain | 0 | M | No | Signal transduction protein |
| Methoprene-tolerant protein | 1 | PAS domain | 0 | / | No | Unknown |
| Salivap-5 | 1 | / | 0 | / | No | Unknown |
| Cchamide 1 | 1 | / | 1 | S | Yes | Unknown |
| Uncharacterized protein LOC105672384 | 1 | Putative peptidase (DUF1758) | 0 | / | No | Unknown |
| Uncharacterized protein LOC105692561 | 1 | / | 3 | / | No | Unknown |
| Protein Daple | 1 | / | 0 | / | No | Unknown |
| Rhogtpase | 1 | BTB/POZ domain | 0 | / | No | Unknown |
| Uncharacterized protein LOC105149357 isoform X1 | 1 | / | 0 | / | No | Unknown |
| Collagen alpha-1(III) chain | 1 | / | 0 | / | No | Unknown |
| Glutamate receptor ionotropic | 1 | / | 0 | / | No | Unknown |
| Protein retinal degeneration B isoform X3 | 1 | / | 0 | / | No | Unknown |
| Hypothetical protein L798_11130 | 1 | / | 0 | M | No | Unknown |
| Triple functional domain | 1 | / | 0 | / | No | Unknown |
| Uncharacterized protein LOC100680511 | 1 | / | 0 | / | No | Unknown |
| Hypothetical protein TcasGA2_TC010304 | 1 | / | 0 | / | No | Unknown |
| Hypothetical protein | 1 | / | 0 | S | Yes | Unknown |
| Uncharacterized protein LOC103508838 isoform X1 | 1 | / | 0 | / | No | Unknown |
| Uncharacterized protein KIAA1143 homolog | 1 | Domain of unknown function (DUF4604) | 0 | M | No | Unknown |
| Hypothetical protein L798_03213 | 1 | / | 0 | / | No | Unknown |
| Glucocorticoid-induced transcript 1 protein-like | 1 | Protein Family FAM117 | 0 | M | No | Unknown |
a The unique matched peptides are shown in S3 Table.
b Protein domains were determined by Pfam version 31.0 (http://pfam.xfam.org/)).
c THMHH Server v. 2.0 (http://www.cbs.dtu.dk/services/TMHMM/) was used to predict transmembrane helices in proteins.
d Subcelluar localization was predicted by TargetP 1.1 Server (http://www.cbs.dtu.dk/services/TargetP/). M stands for mitochondrial targeting peptide. S stands for secretory pathway signal peptide. “/” stands for other.
eSignal peptide was determined by SignalP 4.1 Server (http://www.cbs.dtu.dk/services/SignalP/).