REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
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Antibodies | ||
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Mouse monoclonal anti-Myc(9B11) | Cell Signaling Technology | #2276;RRID:AB_2148465 |
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Mouse monoclonal anti-GFP (JL-8) | Clontech | 632381; RRID: AB_2313808 |
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Rat monoclonal anti-Tubulin (YL1/2) | abcam | Ab6;RRID:AB_343284 |
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Rat monoclonal anti-HA | Roche | 11867423001; RRID:AB_390919 |
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Mouse monoclonal anti-Ubiquitin (P4D1) | Cell signaling | 3936; RRID:AB_331292 |
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Rabbit polyclonal anti-BZR1 | This paper | homemade |
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Rabbit polyclonal anti-BIN2 | This paper | Homemade |
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Chemicals, Peptides, and Recombinant Proteins | ||
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Brassinolide (BL) | Wako | 635-00811 |
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Propiconazole (PPZ) | Control Solutions | N/A |
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Bikinin | ChemBridge | 5122035 |
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lambda protein phosphatase | NEB | P0753 |
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MG132 | Sigma-Aldrich | C2211 |
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Critical Commercial Assays | ||
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GFP-nAb Magnetic Agarose Kit | Allele Biotech | ABP-NABGFPXK20 |
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Auto-ubiquitinylation kit | Enzo life science | BML-UW0970-0001 |
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Protein A/G plus agarose | Pierce | 20423 |
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TnT quick couple transcription/translation system | Promega | L1170 |
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Spectrum plant total RNA kit | Sigma-Aldrich | STRN250 |
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M-MLV reverse transcriptase | ThermoFisher | 28025013 |
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Experimental Models: Organisms/Strains | ||
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Arabidopsis: Col-0, Ws | ABRC | |
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Arabidopsis: bzr1-1D | Wang et al., 2002 | N/A |
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Arabidopsis bin2-1, bin2-3bil1bil3 |
Li and Nam.,
2002; Yan et al., 2009 |
N/A |
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Arabidopsis: dwf4 | Choe et al., 1998 | N/A |
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Arabidopsis: bri1–116 | Wang et al., 2001 | N/A |
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Arabidopsis: BIN2-ox | Kim et al., 2010 | N/A |
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Arabidopsis: KIB1-ox, kib1-cs, kib1-1,
KIB1C-ox,KIB1-YFP/bzr1-1D, KIB1-ox/BIN2-ox, KIB1-ox/bin2-1, KIB1::KIB1-YFP, KIB1-GUS |
This paper | N/A |
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Arabidopsis: kib1–2 | ABRC | SAIL_1230_F06 |
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Oligonucleotides | ||
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Primers for KIB1qRT-PCR | ||
F: ATTCGATACTTGCCGTGGAC | This paper | Customer order |
R: GGATGAGCCATGGAGTTGTT | ||
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Primers for KIB2qRT-PCR | ||
F: TCCAGCTGCTTGAGAGTTGA | This paper | Customer order |
R: ACAGGCTGCTTTGAATCGTC | ||
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Primers for KIB3qRT-PCR | ||
F: GGTTTTTCCCCACTTTTGGT | This paper | Customer order |
R: CGTCACTGGTTTTGTCCCTT | ||
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Primers for KIB4qRT-PCR | ||
F: GGAGTCGTTGTTTGGCAAGT | This paper | Customer order |
R: AACCACAGTCACCGGTTCTC | ||
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Primers for AT3G03726qRT-PCR | ||
F: CAACGATTTCCACGTTTTGA | This paper | Customer order |
R: CCACAAAACCGCAGTGTCTA | ||
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Primers for AT4G12800qRT-PCR | ||
F: TCCGACAAGACAACATTCCA | This paper | Customer order |
R: GGTACGGTATCCAGGGAGGT | ||
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Primers for BIN2qRT-PCR | ||
F:TCTGCTGGTTGTGTTCTTGC | This paper | Customer order |
R: AAGATCTTGTGCCAGGGATG | ||
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Primers for PP2AqRT-PCR | ||
F: TATCGGATGACGATTCTTCGTGCAG | This paper | Customer order |
R: GCTTGGTCGACTATCGGAATGAGAG | ||
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Primers for KIB1 promoter cloning | ||
F:CACCTCTCCATCTAACACACTAAGCACA | This paper | Customer order |
R: GGCCAAGTAAAGACCTAGAAACAT | ||
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Primers for full length KIB1 cloning | ||
F: CACCATGACACATAAGAAGCAGAAGAAG | This paper | Customer order |
R: TTAAAGCAACCATTTTGAAGTA | ||
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Primer for KIB1N cloning | ||
R: ACTATGTAGGTTTTGTTTTCATGAGG | This paper | Customer order |
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Primer for KIB1C cloning | ||
F:CACCATGAAGAGACCTCGGGTTTGATTTAGTTA | This paper | Customer order |
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Primers for full length KIB2 cloning | ||
F: CACCATGGCGCCTCTCAACTCTCA | This paper | Customer order |
R: AAGCAACCATTTTCCACCAA | ||
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Primers for full length ASK1 cloning | ||
F: CACCATGTCTGCGAAGAAGATTGTGTTGAA | This paper | Customer order |
R: TTCAAAAGCCCATTGGTTCTCTCTGCG | ||
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Recombinant DNA | ||
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KIB1::KIB1-YFP | This paper | N/A |
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35S::KIB1-YFP | This paper | N/A |
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35S::KIB1-Mys | This paper | N/A |
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35s::KIB1C-YFP | This paper | N/A |
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KIB1-GUS | This paper | N/A |
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BD-KIB1F, BD-KIB1C, BD-KIB1N, AD-KIB1, AD-KIB2, AD-ASK1 | This paper | N/A |
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AD-BIN2, AD-bin2-1, AD-mBIN2, BD-AtASK11, BD-AtASK12, BD-AtASK13, BD-AtASK22, BD-AtASK23 | Kim et al., 2009 | N/A |
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AD-BIN2-GFP | This paper | N/A |
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AD-KIB1-YFP | This paper | N/A |
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pXDGATcy86 | Ding et al., 2007 | N/A |
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pGADT7-GW | Lu et al., 2010 | N/A |
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pMDC164 | Curtis et al., 2003 | N/A |
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Software and Algorithms | ||
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Clustal Omega | Sievers et al., 2011 | http://www.clustal.org/omega/ RRID:SCR_001591 |
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ImageJ | Schneider et al., 2012 | https://imagej.net/ImageJ RRID:SCR_003070 |
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Fiji | Schindelin et al., 2012 | https://imagej.net/Fiji RRID:SCR_002285 |
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PHYLIP | Felsenstein, 1989 | http://evolution.genetics .washington.edu/phylip/ RRID:SCR_006244 |