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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Mol Cell. 2017 Jun 1;66(5):648–657.e4. doi: 10.1016/j.molcel.2017.05.012

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER

Antibodies

Mouse monoclonal anti-Myc(9B11) Cell Signaling Technology #2276;RRID:AB_2148465

Mouse monoclonal anti-GFP (JL-8) Clontech 632381; RRID: AB_2313808

Rat monoclonal anti-Tubulin (YL1/2) abcam Ab6;RRID:AB_343284

Rat monoclonal anti-HA Roche 11867423001; RRID:AB_390919

Mouse monoclonal anti-Ubiquitin (P4D1) Cell signaling 3936; RRID:AB_331292

Rabbit polyclonal anti-BZR1 This paper homemade

Rabbit polyclonal anti-BIN2 This paper Homemade

Chemicals, Peptides, and Recombinant Proteins

Brassinolide (BL) Wako 635-00811

Propiconazole (PPZ) Control Solutions N/A

Bikinin ChemBridge 5122035

lambda protein phosphatase NEB P0753

MG132 Sigma-Aldrich C2211

Critical Commercial Assays

GFP-nAb Magnetic Agarose Kit Allele Biotech ABP-NABGFPXK20

Auto-ubiquitinylation kit Enzo life science BML-UW0970-0001

Protein A/G plus agarose Pierce 20423

TnT quick couple transcription/translation system Promega L1170

Spectrum plant total RNA kit Sigma-Aldrich STRN250

M-MLV reverse transcriptase ThermoFisher 28025013

Experimental Models: Organisms/Strains

Arabidopsis: Col-0, Ws ABRC

Arabidopsis: bzr1-1D Wang et al., 2002 N/A

Arabidopsis bin2-1, bin2-3bil1bil3 Li and Nam., 2002;
Yan et al., 2009
N/A

Arabidopsis: dwf4 Choe et al., 1998 N/A

Arabidopsis: bri1–116 Wang et al., 2001 N/A

Arabidopsis: BIN2-ox Kim et al., 2010 N/A

Arabidopsis: KIB1-ox, kib1-cs, kib1-1, KIB1C-ox,KIB1-YFP/bzr1-1D,
KIB1-ox/BIN2-ox, KIB1-ox/bin2-1, KIB1::KIB1-YFP, KIB1-GUS
This paper N/A

Arabidopsis: kib1–2 ABRC SAIL_1230_F06

Oligonucleotides

Primers for KIB1qRT-PCR
F: ATTCGATACTTGCCGTGGAC This paper Customer order
R: GGATGAGCCATGGAGTTGTT

Primers for KIB2qRT-PCR
F: TCCAGCTGCTTGAGAGTTGA This paper Customer order
R: ACAGGCTGCTTTGAATCGTC

Primers for KIB3qRT-PCR
F: GGTTTTTCCCCACTTTTGGT This paper Customer order
R: CGTCACTGGTTTTGTCCCTT

Primers for KIB4qRT-PCR
F: GGAGTCGTTGTTTGGCAAGT This paper Customer order
R: AACCACAGTCACCGGTTCTC

Primers for AT3G03726qRT-PCR
F: CAACGATTTCCACGTTTTGA This paper Customer order
R: CCACAAAACCGCAGTGTCTA

Primers for AT4G12800qRT-PCR
F: TCCGACAAGACAACATTCCA This paper Customer order
R: GGTACGGTATCCAGGGAGGT

Primers for BIN2qRT-PCR
F:TCTGCTGGTTGTGTTCTTGC This paper Customer order
R: AAGATCTTGTGCCAGGGATG

Primers for PP2AqRT-PCR
F: TATCGGATGACGATTCTTCGTGCAG This paper Customer order
R: GCTTGGTCGACTATCGGAATGAGAG

Primers for KIB1 promoter cloning
F:CACCTCTCCATCTAACACACTAAGCACA This paper Customer order
R: GGCCAAGTAAAGACCTAGAAACAT

Primers for full length KIB1 cloning
F: CACCATGACACATAAGAAGCAGAAGAAG This paper Customer order
R: TTAAAGCAACCATTTTGAAGTA

Primer for KIB1N cloning
R: ACTATGTAGGTTTTGTTTTCATGAGG This paper Customer order

Primer for KIB1C cloning
F:CACCATGAAGAGACCTCGGGTTTGATTTAGTTA This paper Customer order

Primers for full length KIB2 cloning
F: CACCATGGCGCCTCTCAACTCTCA This paper Customer order
R: AAGCAACCATTTTCCACCAA

Primers for full length ASK1 cloning
F: CACCATGTCTGCGAAGAAGATTGTGTTGAA This paper Customer order
R: TTCAAAAGCCCATTGGTTCTCTCTGCG

Recombinant DNA

KIB1::KIB1-YFP This paper N/A

35S::KIB1-YFP This paper N/A

35S::KIB1-Mys This paper N/A

35s::KIB1C-YFP This paper N/A

KIB1-GUS This paper N/A

BD-KIB1F, BD-KIB1C, BD-KIB1N, AD-KIB1, AD-KIB2, AD-ASK1 This paper N/A

AD-BIN2, AD-bin2-1, AD-mBIN2, BD-AtASK11, BD-AtASK12, BD-AtASK13, BD-AtASK22, BD-AtASK23 Kim et al., 2009 N/A

AD-BIN2-GFP This paper N/A

AD-KIB1-YFP This paper N/A

pXDGATcy86 Ding et al., 2007 N/A

pGADT7-GW Lu et al., 2010 N/A

pMDC164 Curtis et al., 2003 N/A

Software and Algorithms

Clustal Omega Sievers et al., 2011 http://www.clustal.org/omega/
RRID:SCR_001591

ImageJ Schneider et al., 2012 https://imagej.net/ImageJ
RRID:SCR_003070

Fiji Schindelin et al., 2012 https://imagej.net/Fiji
RRID:SCR_002285

PHYLIP Felsenstein, 1989 http://evolution.genetics
.washington.edu/phylip/
RRID:SCR_006244