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. Author manuscript; available in PMC: 2018 May 4.
Published in final edited form as: Cell Rep. 2018 Apr 17;23(3):918–929. doi: 10.1016/j.celrep.2018.03.093

Figure 6. GPBP1 Loss Causes Upregulation of the Homologous Recombination Pathway.

Figure 6

(A) Gene set enrichment analysis of homologous recombination pathway genes using RNA-seq data from GPBP1-knockdown MCF10A cells treated with 0.5 μM BMN673 or DMSO for 24 hr.

(B) Heatmap representation of expression of HR pathway genes differentially expressed in the presence of BMN673. TPM, transcripts per kilobase million.

(C and D) Quantification of gamma-H2AX foci (C) and RAD51 recruitment (D) after treatment with 500 nM of BMN673 in the presence of the indicated gene knockdowns in MCF10A cells.

(E and F) Quantification of gamma-H2AX foci (E) and RAD51 recruitment (F) after treatment with 50 nM of BMN673 in the presence of the indicated gene knockdowns in SUM149PT cells. NT4 was the non-targeting control.

Error bars indicate SEM.

See also Figure S4.