Figure 5. Fully mature adult-born DA neurons are small and never possess an AIS.

(A) Schematic representation of the experimental strategy: pregnant wild-type mice were injected with a single dose of BrdU, and their tissue was collected 4 months later to allow full maturation of adult-born neurons. (B) Example image of adult tissue perfused 4 months post-BrdU injection, stained with antibodies against BrdU (green), TH (blue) and the AIS marker pIκBα (magenta). Asterisk indicates a BrdU +DA cell (TH+/pIκBα-), triangles indicate AIS start and end positions; the arrow indicates a neighbouring large AIS +DA neuron that is BrdU-negative.