Table 7.
Stability study of optimized formulation LPNPs-8.
| Formulation LPNPs-8 | |||||
|---|---|---|---|---|---|
| Time (days) | Microscopic observation | % Encapsulation efficiency | Particle size (nm) | PDI | Zeta potential (mV) |
| At 4 °C/60 ± 5% RH (n = 3) | |||||
| Initial | Smooth spherical vesicles | 89.72 ± 0.23 | 178.6 ± 3.7 | 0.206 ± 0.36 | +41.5 ± 3.4 |
| 7 | Smooth spherical vesicles | 87.11 ± 2.51 | 189.7 ± 1.87 | 0.227 ± 0.78 | +39.43 ± 2.2 |
| 15 | Smooth spherical vesicles | 84.62 ± 1.98 | 197.45 ± 5.2 | 0.279 ± 0.32 | +36.16 ± 2.5 |
| 30 | Smooth spherical vesicles | 81.82 ± 3.41 | 205.8 ± 3.36 | 0.305 ± 0.63 | +34.08 ± 4.5 |
| 60 | Rough spherical vesicles | 80.03 ± 6.2 | 219 ± 4.32 | 0.328 ± 0.78 | +33.62 ± 3.6 |
| 90 | Rough spherical vesicles | 79.29 ± 1.94 | 274 ± 3.66 | 0.353 ± 0.71 | +30.3 ± 3.33 |
| At 25 °C/60 ± 5% RH (n = 3) | |||||
| Initial | Smooth spherical vesicles | 89.72 ± 0.23 | 178.6 ± 3.7 | 0.206 ± 0.36 | +41.5 ± 3.4 |
| 7 | Smooth spherical vesicles | 84.54 ± 3.54 | 187.04 ± 1.7 | 0.225 ± 0.74 | +39.66 ± 3.2 |
| 15 | Rough spherical vesicles | 80.65 ± 5.09 | 205.36 ± 4.6 | 0.271 ± 0.54 | +35.43 ± 1.98 |
| 30 | Rough spherical vesicles | 78.88 ± 2.76 | 235.4 ± 2.54 | 0.334 ± 0.19 | +32.76 ± 1.55 |
| 60 | Agglomerate vesicles | 76.54 ± 3.33 | 275.4 ± 4.05 | 0.402 ± 0.72 | +29.12 ± 4.5 |
| 90 | Agglomerate vesicles | 72.42 ± 2.54 | 370.7 ± 3.43 | 0.471 ± 0.29 | +23.09 ± 3.4 |
Data represent mean ± SD; n = 3.