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. Author manuscript; available in PMC: 2018 May 4.
Published in final edited form as: J Am Chem Soc. 2018 Jan 31;140(6):2020–2023. doi: 10.1021/jacs.7b12766

Figure 3.

Figure 3

Fluorescence of 3 in brain slices containing the dorsal cochlear nucleus (DCN) after photoactivation. (A) 4× image of the DCN slice (left), with overlaid 8 × 8 photostimulation grid (40 μm spacing). 20× image of the DCN (right) with the overlaid grid. The grid was photostimulated 5 times with 1 ms pulses (355 nm, ~5.5 mW). (B) Heat map of normalized fluorescent signals. ΔF/F is the fluorescence change after photostimulation divided by the initial fluorescence. (C) Comparison of the average photoactivated fluorescence of sensor 3 in the molecular layer versus the deep layer (regions outlined by yellow boxes in B, n = 6, p < 0.0001, unpaired t test).