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. Author manuscript; available in PMC: 2019 May 3.
Published in final edited form as: Mol Cell. 2018 Apr 19;70(3):553–564.e9. doi: 10.1016/j.molcel.2018.03.014

Figure 1. CapZyme-Seq, a high-throughput-sequencing method to detect NCIN-capped RNA.

Figure 1

A. Structures of ATP and adenosine-containing NCINs NAD+, NADH, dpCoA, and FAD. Red, identical atoms.

B. Processing of RNA 5′-ends by NudC, Rai1, and Rpp. Red, common moiety of each 5′-end; black, distinct moiety of each 5′-end; grey, remainder of RNA.

C. Products of processing of NCIN-capped RNA 5′-ends by NudC, Rai1, and Rpp.

D. CapZyme-Seq procedure. Grey, RNA; purple, 5′ adaptor; red, 3′ adaptor; black cDNA.