A. Structures of ATP and adenosine-containing NCINs NAD+, NADH, dpCoA, and FAD. Red, identical atoms.
B. Processing of RNA 5′-ends by NudC, Rai1, and Rpp. Red, common moiety of each 5′-end; black, distinct moiety of each 5′-end; grey, remainder of RNA.
C. Products of processing of NCIN-capped RNA 5′-ends by NudC, Rai1, and Rpp.
D. CapZyme-Seq procedure. Grey, RNA; purple, 5′ adaptor; red, 3′ adaptor; black cDNA.