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. Author manuscript; available in PMC: 2019 May 3.
Published in final edited form as: Cell. 2018 Apr 5;173(4):934–945.e12. doi: 10.1016/j.cell.2018.02.062

Figure 6. Most close-fusions release NPY-EGFP rapidly and completely as non-close-fusions.

Figure 6

(A–D) F647 (A647 spot fluorescence), FNPY (NPY-EGFP spot fluorescence), A647 spot WH, and A647/NPY-EGFP confocal XY-images at times indicated with lines for various fusion modes and rates of releasing NPY-EGFP. A, close-fusion with rapid release; B, non-close-fusion, including stay-fusion (left) and shrink-fusion (right) with rapid release; C, two stay-fusion spots with slow release; D, close-fusion (left) and stay-fusion (right) with no or partial release of NPY-EGFP.

(E) Cumulative frequency (normalized) of 20–80% FNPY decay time for close-fusion (95 spots, 28 cells) and non-close-fusion (123 spots, 28 cells, confocal XY/Zfix imaging). ‘>30 s’: partial or no NPY-EGFP release.