REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
DMEM medium	Gibco	Cat#11885092
Fetal bovine serum	Gibco	Cat#10082147
Poly-L-lysine	Sigma	P4832; CAS: 25988-63-0
Laminin	Sigma	L2020; CAS: 114956-81-9
Atto 532	Sigma	88793
Alexa 647	Sigma	A20502
Trypsin inhibitor	Sigma	T9253; CAS: 9035-81-8
Bovine serum albumin	Sigma	A2153; CAS: 9048-46-8
mCLING-Atto 488	Synaptic Systems	Cat#710 006AT3
FFN511	Abcam	Cat#ab120331
Embed-812	EMS	Cat#13940
Collagenase P	Sigma	COLLP-RO Roche 11249002001
Dynasore	Tocris Bioscience	Cat#2897; CAS: 30448-55-3
Latrunculin A	Tocris Bioscience	Cat#3973; CAS: 76343-93-6
Critical Commercial Assays
Basic Primary Neurons Nucleofector Kit	Lonza	Cat#VVPI-1003
Experimental Models: Primary Cell Culture
Bovine adrenal chromaffin cell	J. W. Treuth and Sons, Inc.	Adrenal Glands
Recombinant DNA
PH-EGFP	Várnai P. and Balla T. 1998	Addgene Plasmid #51407
PH-mCherry	Chisari et al., 2009	Addgene Plasmid #36075
mNeonGreen	Allele Biotechnology	ABP-FP-MNEONSB
PH-mNeonGreen	This paper	N/A
NPY-EGFP	Taraska et al., 2003	Addgene Plasmid #74629
Dynamin 2-EGFP	He K. et al., 2015	Addgene Plasmid #34686
mTurquoise2	Goedhard et al., 2012	Addgene Plasmid #54843
NPY-mTurquoise2	This paper	N/A
Dynamin 2-mTurquoise2	This paper	N/A
Dynamin 1-K44A-mRFP	Hynes et al., 2004	Addgene Plasmid #55795
Lifeact-TagGFP2	Ibidi	Cat#60112
Software and Algorithms
Huygens Professional	Scientific Volume Imaging	https://svi.nl/Huygens-Professional
Image J	NIH	https://imagej.nih.gov/ij/
LAS X	Leica	https://www.leicabiosystems.com/
Igor Pro	WaveMetrics	http://www.wavemetrics.com/