Chemicals, Peptides, and Recombinant Proteins |
DMEM medium |
Gibco |
Cat#11885092 |
Fetal bovine serum |
Gibco |
Cat#10082147 |
Poly-L-lysine |
Sigma |
P4832; CAS: 25988-63-0 |
Laminin |
Sigma |
L2020; CAS: 114956-81-9 |
Atto 532 |
Sigma |
88793 |
Alexa 647 |
Sigma |
A20502 |
Trypsin inhibitor |
Sigma |
T9253; CAS: 9035-81-8 |
Bovine serum albumin |
Sigma |
A2153; CAS: 9048-46-8 |
mCLING-Atto 488 |
Synaptic Systems |
Cat#710 006AT3 |
FFN511 |
Abcam |
Cat#ab120331 |
Embed-812 |
EMS |
Cat#13940 |
Collagenase P |
Sigma |
COLLP-RO Roche 11249002001 |
Dynasore |
Tocris Bioscience |
Cat#2897; CAS: 30448-55-3 |
Latrunculin A |
Tocris Bioscience |
Cat#3973; CAS: 76343-93-6 |
Critical Commercial Assays |
Basic Primary Neurons Nucleofector Kit |
Lonza |
Cat#VVPI-1003 |
Experimental Models: Primary Cell Culture |
Bovine adrenal chromaffin cell |
J. W. Treuth and Sons, Inc. |
Adrenal Glands |
Recombinant DNA |
PH-EGFP |
Várnai P. and Balla T. 1998 |
Addgene Plasmid #51407 |
PH-mCherry |
Chisari et al., 2009 |
Addgene Plasmid #36075 |
mNeonGreen |
Allele Biotechnology |
ABP-FP-MNEONSB |
PH-mNeonGreen |
This paper |
N/A |
NPY-EGFP |
Taraska et al., 2003 |
Addgene Plasmid #74629 |
Dynamin 2-EGFP |
He K. et al., 2015 |
Addgene Plasmid #34686 |
mTurquoise2 |
Goedhard et al., 2012 |
Addgene Plasmid #54843 |
NPY-mTurquoise2 |
This paper |
N/A |
Dynamin 2-mTurquoise2 |
This paper |
N/A |
Dynamin 1-K44A-mRFP |
Hynes et al., 2004 |
Addgene Plasmid #55795 |
Lifeact-TagGFP2 |
Ibidi |
Cat#60112 |
Software and Algorithms |
Huygens Professional |
Scientific Volume Imaging |
https://svi.nl/Huygens-Professional |
Image J |
NIH |
https://imagej.nih.gov/ij/ |
LAS X |
Leica |
https://www.leicabiosystems.com/ |
Igor Pro |
WaveMetrics |
http://www.wavemetrics.com/ |