(A) Western blot analysis of EZH2, H3K27me3, and total histone H3 levels in SU-DHL-4 (left, PRC2-independent) and KARPAS-422 (right, PRC2-independent) DLBCL cells with control (EV), EZH2 knockdown (shEZH2), and EZH2 rescue conditions, as indicated on the right side. Gapdh was used as a loading control. EV, empty vector.
(B) Proliferation assays for SU-DHL-4 (left) and KARPAS-422 (right) cells under the abovementioned conditions. 10,000 cells were seeded in 6-well plates and cell numbers were counted after 2, 4, and 6 days. The culture medium was replenished every two days. Cell numbers are shown as Mean ± SD (n=3 for each data point).