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. Author manuscript; available in PMC: 2019 May 3.
Published in final edited form as: Mol Cell. 2018 Apr 19;70(3):422–434.e6. doi: 10.1016/j.molcel.2018.03.020

Figure 5. Disrupting allosteric activation of PRC2 impairs both H3K27me3 levels in EZH2Y646X-mutant DLBCLs and proliferation of PRC2-addicted cells.

Figure 5

(A) Western blot analysis of EZH2, H3K27me3, and total histone H3 levels in SU-DHL-4 (left, PRC2-independent) and KARPAS-422 (right, PRC2-independent) DLBCL cells with control (EV), EZH2 knockdown (shEZH2), and EZH2 rescue conditions, as indicated on the right side. Gapdh was used as a loading control. EV, empty vector.

(B) Proliferation assays for SU-DHL-4 (left) and KARPAS-422 (right) cells under the abovementioned conditions. 10,000 cells were seeded in 6-well plates and cell numbers were counted after 2, 4, and 6 days. The culture medium was replenished every two days. Cell numbers are shown as Mean ± SD (n=3 for each data point).