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. Author manuscript; available in PMC: 2019 May 15.
Published in final edited form as: Bioorg Med Chem. 2018 Mar 19;26(9):2345–2353. doi: 10.1016/j.bmc.2018.03.028

Figure 2.

Figure 2

Proteins used to screen compounds for REV1/UbPCNA interaction inhibition. A: REV1 (1251 AA) and its truncated variant (REV1Δ, 695 AA), which was used to screen chemical compounds for inhibiting REV1/UbPCNA interaction. Dotted lines indicate the truncation to generate REV1Δ. B: In vitro production of K164-UbPCNA tagged with a biotin on the ubiquitin N-terminus. The reaction mixtures were analyzed by Coomassie-staining (left) or immunoblotting for pan-PCNA (right, PC-10 antibody) to confirm the UbPCNA bands.