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. Author manuscript; available in PMC: 2019 May 3.
Published in final edited form as: Cell. 2018 Mar 22;173(4):920–933.e13. doi: 10.1016/j.cell.2018.02.055

Figure 6. IRF8 confers protection against S. Typhimurium in vivo.

Figure 6

(A) Body weight of 8-week-old WT (n=13), Irf8−/− (n=7), Irf8+/− (n=6) and Nlrc4−/− (n=9) mice infected intraperitoneally with 103 CFU of S. Typhimurium.

(B) Survival of WT (n=13), Irf8−/− (n=7), Irf8+/− (n=6), Naip5−/− (n=9) and Nlrc4−/− mice (n=9) infected as (A).

(C and D) Bacterial burden in the spleen and liver of WT (n=20), Irf8−/− (n=15), Nlrc4−/− (n=15) and Naip5−/−(n=9) mice on day 3 after infection as (A).

(E and F) Analysis of IL-18 in the spleen and liver collected from WT (n=10), Irf8−/− (n=6), Nlrc4−/− (n=10) and Naip5−/− (n=6) mice on day 3 after infection as (A).

(G–L) RT-PCR analysis of genes encoding NAIPs, NLRC4, and IRF8 in the spleen collected from WT and Irf8−/− mice before infection (day 0) and after infection (day 3) as (A), presented relative to that of the gene encoding HPRT.

NS, not significant; *P < 0.05, ***P < 0.0001 and ****P < 0.0001 (log-rank test [B], ANOVA with Dunn’s multiple comparisons test [C–F], or Two-way ANOVA with Tukey’s multiple comparisons test [G–L]). Data are from 2 experiments.