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Temperature (a) and pH (b) dependence of endo-glucanase activity for wild-type CelStrep and mutants. For the optimal temperature endo-glucanase activity was measured at pH 6 and temperatures ranging from 40 to 80 °C, using AZO-CMC as substrate. For the optimal pH determination, the endo-glucanase activity was measured in Na acetate buffer at pH ranging from 4 to 6, using AZO-CMC as substrate
