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. 2017 Jul 1;3(1):87–96. doi: 10.1016/j.bioactmat.2017.06.003

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6 — Cumulative BSA release profiles of various (a) nanogel/SiO₂ NPs, Fe₃O₄-loaded nanogel/SiO₂ NPs triggered by (b) a 30 min high-frequency alternating magnetic fields (HFMF) at the 180 min mark, and (c) a 10 min HFMF stimulation applied at the three specific times (at 3, 5 and 7 h), and nanogel/SiO₂/PAA NPs at (d) pH 1.2 and (e) pH 7.4. (f) ALP activity after 3, 6 and 9 days of culture with various composite biofilms. Legend: 4h or 10h + rhBMP-2 = film fabricated using nanogel/SiO₂/PAA/HA NPs based on the 4h or 10h nanogel template loaded with rhBMP-2; HAp + rhBMP-2 = positive control of PLGA/HA film loaded with rhBMP-2; HAp = negative control of PLGA/HA film without rhBMP-2.